How can posthumanism help us reframe AI-mediated literacy practices? And what implications does such reframing have for cultivating AI literacy in language and literacy education? This article explores these two imperative questions through a case study analyzing two multilingual undergraduate students’ meaning-making and meaning-negotiation intra-actions with AI technologies in a writing classroom. The case study reveals a productive tension between these students’ experiments with posthumanist literacy and their entrenched humanistic assumptions. Ultimately, through the case study, the authors hope to demonstrate that reframing and re-engaging with AI literacy through a posthumanist lens may offer students and educators a relational approach to developing and cultivating AI literacy.